ProSci

ARHGAP5 Antibody

Product Code:
 
PSI-19-096
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using ARHGAP5 antibody (19-096) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 3
Immunohistochemistry of paraffin-embedded rat brain using ARHGAP5 antibody (19-096) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of HeLa cells using ARHGAP5 antibody (19-096) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ARHGAP5 antibody (19-096) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using ARHGAP5 antibody (19-096) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ARHGAP5 antibody (19-096) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-19-096-100uL100 uL£565.00
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This product comes from: United States.
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Further Information

Additional Names:
GFI2, RhoGAP5, p190-B, p190BRhoGAP, rho GTPase-activating protein 5, growth factor independent 2, p100 RasGAP-associated p105 protein, p105 RhoGAP, rho-type GTPase-activating protein 5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human ARHGAP5 (NP_001164.2).
NCBI Gene ID #:
394
NCBI Official Name:
Rho GTPase activating protein 5
NCBI Official Symbol:
ARHGAP5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 185kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q13017
User NOte:
Optimal dilutions for each application to be determined by the researcher.