ProSci

ATP6V1E1 Antibody

Product Code:
 
PSI-19-150
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody (19-150) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.

Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody (19-150) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.

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CodeSizePrice
PSI-19-150-100uL100 uL£565.00
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This product comes from: United States.
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Further Information

Additional Names:
ARCL2C, ATP6E, ATP6E2, ATP6V1E, P31, Vma4, V-type proton ATPase subunit E 1, ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1, H(+)-transporting two-sector ATPase, 31kDa subunit, H+-transporting ATP synthase chain E, vacuolar, V-ATPase 31 kDa subunit, V-ATPase subunit E 1, V-ATPase, subunit E, vacuolar proton pump subunit E 1
Application Note:
WB: 1:1000 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 77-226 of human ATP6V1E1 (NP_001687.1).
NCBI Gene ID #:
529
NCBI Official Name:
ATPase H+ transporting V1 subunit E1
NCBI Official Symbol:
ATP6V1E1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P36543
User NOte:
Optimal dilutions for each application to be determined by the researcher.