ProSci

CRYM Antibody

Product Code:
 
PSI-19-168
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using CRYM antibody (19-168) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Western blot analysis of extracts of rat brain, using CRYM antibody (19-168) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 30s.
3 / 4
Immunohistochemistry of paraffin-embedded rat brain using CRYM antibody (19-168) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse brain using CRYM antibody (19-168) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using CRYM antibody (19-168) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of rat brain, using CRYM antibody (19-168) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CRYM antibody (19-168) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CRYM antibody (19-168) at dilution of 1:100 (40x lens).

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PSI-19-168-100uL100 uL£565.00
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Further Information

Additional Names:
CRYM, Crystallin, mu, Ketimine reductase, Mu-crystallin homolog, THBP, DFNA40
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human CRYM (NP_001879.1).
NCBI Gene ID #:
1428
NCBI Official Name:
crystallin, mu
NCBI Official Symbol:
CRYM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Neuroscience, Signal Transduction
Swissprot #:
Q14894
User NOte:
Optimal dilutions for each application to be determined by the researcher.