Antibody Clonality:
Polyclonal
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
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![Western blot analysis of extracts of various cell lines, using PSMB6 antibody (19-259) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s. Western blot analysis of extracts of various cell lines, using PSMB6 antibody (19-259) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.](https://www.prosci-inc.com/static-images/19-259_1.jpg)
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![Immunofluorescence analysis of HeLa cells using PSMB6 Polyclonal Antibody (19-259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Immunofluorescence analysis of HeLa cells using PSMB6 Polyclonal Antibody (19-259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](https://www.prosci-inc.com/static-images/19-259_2.jpg)
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This product comes from:
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Further Information
DELTA, LMPY, Y, proteasome subunit beta type-6, PSY large multifunctional protease Y, macropain delta chain, multicatalytic endopeptidase complex delta chain, proteasome (prosome, macropain) subunit, beta type, 6, proteasome catalytic subunit 1, proteasome delta chain, proteasome subunit Y, proteasome subunit delta
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Recombinant fusion protein containing a sequence corresponding to amino acids 35-239 of human PSMB6 (NP_002789.1).
5694
proteasome subunit beta 6
PSMB6
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 23kDa
Affinity purification
Cell Cycle
P28072
Optimal dilutions for each application to be determined by the researcher.