ProSci

HSPA13 Antibody

Product Code:
 
PSI-19-289
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of U-87MG cells, using HSPA13 antibody (19-289) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 3
Immunofluorescence analysis of C6 cells using HSPA13 Polyclonal Antibody (19-289) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of L929 cells using HSPA13 Polyclonal Antibody (19-289) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of U-87MG cells, using HSPA13 antibody (19-289) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of C6 cells using HSPA13 Polyclonal Antibody (19-289) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using HSPA13 Polyclonal Antibody (19-289) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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CodeSizePrice
PSI-19-289-100uL100 uL£565.00
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Further Information

Additional Names:
HSPA13, heat shock protein 70kDa family, member 13, STCH, heat shock 70 kDa protein 13, microsomal stress 70 protein ATPase core, microsomal stress-70 protein ATPase core, stress 70 protein chaperone, microsome-associated, 60kD, stress 70 protein chaperon
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of the heat shock protein 70 family and is found associated with microsomes. Members of this protein family play a role in the processing of cytosolic and secretory proteins, as well as in the removal of denatured or incorrectly-folded proteins. The encoded protein contains an ATPase domain and has been shown to associate with a ubiquitin-like protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 165-471 of human HSPA13 (NP_008879.3).
NCBI Gene ID #:
6782
NCBI Official Name:
heat shock protein 70kDa family, member 13
NCBI Official Symbol:
HSPA13
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 65kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P48723
User NOte:
Optimal dilutions for each application to be determined by the researcher.