ProSci

UBE2L3 Antibody

Product Code:
 
PSI-19-297
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 6
Western blot analysis of extracts of various cell lines, using UBE2L3 antibody (19-297) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
2 / 6
Immunohistochemistry of paraffin-embedded rat heart using UBE2L3 antibody (19-297) at dilution of 1:100 (40x lens).
3 / 6
Immunohistochemistry of paraffin-embedded human stomach using UBE2L3 antibody (19-297) at dilution of 1:100 (40x lens).
4 / 6
Immunohistochemistry of paraffin-embedded mouse brain using UBE2L3 antibody (19-297) at dilution of 1:100 (40x lens).
5 / 6
Immunofluorescence analysis of HeLa cells using UBE2L3 Polyclonal Antibody (19-297) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
6 / 6
Immunofluorescence analysis of NIH-3T3 cells using UBE2L3 Polyclonal Antibody (19-297) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using UBE2L3 antibody (19-297) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat heart using UBE2L3 antibody (19-297) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using UBE2L3 antibody (19-297) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using UBE2L3 antibody (19-297) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using UBE2L3 Polyclonal Antibody (19-297) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using UBE2L3 Polyclonal Antibody (19-297) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-19-297-100uL100 uL£565.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4
Application Note:
WB: 1:1000 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 73-212 of human UBE2L3 (NP_001243284.1).
NCBI Gene ID #:
7332
NCBI Official Name:
ubiquitin-conjugating enzyme E2L 3
NCBI Official Symbol:
UBE2L3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 18kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
P68036
User NOte:
Optimal dilutions for each application to be determined by the researcher.