ProSci

UQCRC2 Antibody

Product Code:
 
PSI-19-301
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using UQCRC2 antibody (19-301) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 3
Immunohistochemistry of paraffin-embedded human liver damage using UQCRC2 antibody (19-301) at dilution of 1:100 (40x lens).
3 / 3
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug UQCRC2 antibody (19-301). Western blot was performed from the immunoprecipitate using UQCRC2 antibody (19-301) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using UQCRC2 antibody (19-301) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human liver damage using UQCRC2 antibody (19-301) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug UQCRC2 antibody (19-301). Western blot was performed from the immunoprecipitate using UQCRC2 antibody (19-301) at a dilition of 1:1000.

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PSI-19-301-100uL100 uL£565.00
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Further Information

Additional Names:
Cytochrome b-c1 complex subunit 2, mitochondrial, Complex III subunit 2, Core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCRC2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
The protein encoded by this gene is located in the mitochondrion, where it is part of the ubiquinol-cytochrome c reductase complex (also known as complex III). This complex constitutes a part of the mitochondrial respiratory chain. Defects in this gene are a cause of mitochondrial complex III deficiency nuclear type 5.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 224-453 of human UQCRC2 (NP_003357.2).
NCBI Gene ID #:
7385
NCBI Official Name:
Cytochrome b-c1 complex subunit 2, mitochondrial
NCBI Official Symbol:
UQCRC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
P22695
User NOte:
Optimal dilutions for each application to be determined by the researcher.