ProSci

ARHGEF5 Antibody

Product Code:
 
PSI-19-304
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using ARHGEF5 antibody (19-304) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
2 / 3
Immunohistochemistry of paraffin-embedded mouse liver using ARHGEF5 antibody (19-304) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse kidney using ARHGEF5 antibody (19-304) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using ARHGEF5 antibody (19-304) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse liver using ARHGEF5 antibody (19-304) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ARHGEF5 antibody (19-304) at dilution of 1:100 (40x lens).

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PSI-19-304-100uL100 uL£565.00
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Further Information

Additional Names:
ARHGEF5, Rho guanine nucleotide exchange factor (GEF) 5, DKFZp686N1969, GEF5, P60, TIM, TIM1, guanine nucleotide regulatory protein TIM, oncogene TIM rho guanine nucleotide exchange factor 5, transforming immortalized mammary oncogene
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1248-1597 of human ARHGEF5 (NP_005426.2).
NCBI Gene ID #:
7984
NCBI Official Name:
Rho guanine nucleotide exchange factor (GEF) 5
NCBI Official Symbol:
ARHGEF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 176kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q12774
User NOte:
Optimal dilutions for each application to be determined by the researcher.