ProSci

SFRP2 Antibody

Product Code:
 
PSI-19-630
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using SFRP2 antibody (19-630) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded rat brain using SFRP2 antibody (19-630) at dilution of 1:200 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse heart using SFRP2 antibody (19-630) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using SFRP2 antibody (19-630) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using SFRP2 antibody (19-630) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using SFRP2 antibody (19-630) at dilution of 1:200 (40x lens).

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PSI-19-630-100uL100 uL£565.00
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Further Information

Additional Names:
SFRP2, FRP-2, SARP1, SDF-5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-295 of human SFRP2 (NP_003004.1).
NCBI Gene ID #:
6423
NCBI Official Name:
secreted frizzled-related protein 2
NCBI Official Symbol:
SFRP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 26kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Stem Cell
Swissprot #:
Q96HF1
User NOte:
Optimal dilutions for each application to be determined by the researcher.