ProSci

PSMB10 Antibody

Product Code:
 
PSI-19-672
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of THP-1 cells, using PSMB10 antibody (19-672) at 1:5000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 4
Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody (19-672) at dilution of 1:200 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody (19-672) at dilution of 1:200 (40x lens).
4 / 4
Immunofluorescence analysis of HeLa cells using PSMB10 antibody (19-672). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of THP-1 cells, using PSMB10 antibody (19-672) at 1:5000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody (19-672) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody (19-672) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using PSMB10 antibody (19-672). Blue: DAPI for nuclear staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-19-672-100uL100 uL£565.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
PSMB10, proteasome (prosome, macropain) subunit, beta type, 10, LMP10, MECL1, MGC1665, beta2i, macropain subunit MECl-1, multicatalytic endopeptidase complex subunit MECl-1, proteasome MECl-1, proteasome beta 10 subunit, proteasome catalytic subunit 2i, proteasome subunit MECL1, proteasome subunit beta 7i
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1).
NCBI Gene ID #:
5699
NCBI Official Name:
proteasome (prosome, macropain) subunit, beta type, 10
NCBI Official Symbol:
PSMB10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 29kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Chemokines & Cytokines, Immunology, Innate Immunity
Swissprot #:
P40306
User NOte:
Optimal dilutions for each application to be determined by the researcher.