ProSci

PSMF1 Antibody

Product Code:
 
PSI-19-728
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using PSMF1 Antibody (19-728) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human liver damage using PSMF1 antibody (19-728) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded rat testis using PSMF1 antibody (19-728) at dilution of 1:50 (40x lens).
❮❯

Western blot analysis of extracts of various cell lines, using PSMF1 Antibody (19-728) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using PSMF1 antibody (19-728) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using PSMF1 antibody (19-728) at dilution of 1:50 (40x lens).

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PSI-19-728-100uL100 uL£565.00
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Further Information

Additional Names:
PSMF1, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), PI31, RP4-545L17.1, proteasome inhibitor hP131 subunit, proteasome inhibitor subunit 1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human PSMF1 (NP_006805.2).
NCBI Gene ID #:
9491
NCBI Official Name:
proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
NCBI Official Symbol:
PSMF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q92530
User NOte:
Optimal dilutions for each application to be determined by the researcher.