ProSci

CDC7 Antibody

Product Code:
 
PSI-19-826
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using CDC7 antibody (19-826) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Western blot analysis of extracts of various cell lines, using CDC7 antibody (19-826) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.

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PSI-19-826-100uL100 uL£565.00
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Further Information

Additional Names:
CDC7L1, HsCDC7, Hsk1, huCDC7, cell division cycle 7-related protein kinase, CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1, CDC7-related kinase, cell division cycle 7 homolog, cell division cycle 7-like protein 1, epididymis secretory sperm binding protein
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 275-574 of human CDC7 (NP_001127892.1).
NCBI Gene ID #:
8317
NCBI Official Name:
cell division cycle 7
NCBI Official Symbol:
CDC7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
O00311
User NOte:
Optimal dilutions for each application to be determined by the researcher.