ProSci

POLR2B Antibody

Product Code:
 
PSI-19-948
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using POLR2B antibody (19-948) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 4
Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (19-948) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (19-948) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (19-948) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using POLR2B antibody (19-948) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (19-948) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (19-948) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (19-948) at dilution of 1:100 (40x lens).

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PSI-19-948-100uL100 uL£565.00
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Further Information

Additional Names:
POLR2B, RPB2, POL2RB, hsRPB2, hRPB140
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).
NCBI Gene ID #:
5431
NCBI Official Name:
polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
NCBI Official Symbol:
POLR2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 134kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P30876
User NOte:
Optimal dilutions for each application to be determined by the researcher.