ProSci

ELAVL3 Antibody

Product Code:
 
PSI-22-005
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using ELAVL3 antibody (22-005) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat brain using ELAVL3 antibody (22-005) at dilution of 1:200 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse brain using ELAVL3 antibody (22-005) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using ELAVL3 antibody (22-005) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using ELAVL3 antibody (22-005) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ELAVL3 antibody (22-005) at dilution of 1:200 (40x lens).

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PSI-22-005-100uL100 uL£565.00
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Further Information

Additional Names:
ELAV-like protein 3, Hu-antigen C, HuC, Paraneoplastic cerebellar degeneration-associated antigen, Paraneoplastic limbic encephalitis antigen 21, ELAVL3, HUC, PLE21
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ELAVL3 (NP_001411.2).
NCBI Gene ID #:
1995
NCBI Official Name:
ELAV-like protein 3
NCBI Official Symbol:
ELAVL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q14576
User NOte:
Optimal dilutions for each application to be determined by the researcher.