ProSci

OSM Antibody

Product Code:
 
PSI-22-037
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using OSM Antibody (22-037) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
2 / 4
Immunohistochemistry of paraffin-embedded human prostate using OSM antibody (22-037) at dilution of 1:100 (40x lens).
3 / 4
Immunofluorescence analysis of C6 cells using OSM antibody (22-037) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of L-929 cells using OSM antibody (22-037) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using OSM Antibody (22-037) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human prostate using OSM antibody (22-037) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using OSM antibody (22-037) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L-929 cells using OSM antibody (22-037) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-22-037-100uL100 uL£565.00
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Further Information

Additional Names:
Oncostatin-M, OSM, OSM
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene encodes a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells. This gene and the related gene, leukemia inhibitory factor, also present on chromosome 22, may have resulted from the duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-252 of human OSM (NP_065391.1).
NCBI Gene ID #:
5008
NCBI Official Name:
Oncostatin-M
NCBI Official Symbol:
OSM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Chemokines & Cytokines, Immunology, Innate Immunity
Swissprot #:
P13725
User NOte:
Optimal dilutions for each application to be determined by the researcher.