ProSci

MYLIP Antibody

Product Code:
 
PSI-22-039
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using MYLIP antibody (22-039) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunohistochemistry of paraffin-embedded human prostate using MYLIP antibody (22-039) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using MYLIP antibody (22-039) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using MYLIP antibody (22-039) at dilution of 1:100 (40x lens).

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PSI-22-039-100uL100 uL£565.00
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Further Information

Additional Names:
IDOL, MIR, E3 ubiquitin-protein ligase MYLIP, E3 ubiquitin ligase-inducible degrader of the low density lipoprotein receptor, RING-type E3 ubiquitin transferase MYLIP, band 4.1 superfamily member BZF1, cellular modulator of immune recognition (c-MIR), inducible degrader of the LDL-receptor
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 266-445 of human MYLIP (NP_037394.2).
NCBI Gene ID #:
29116
NCBI Official Name:
myosin regulatory light chain interacting protein
NCBI Official Symbol:
MYLIP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
Q8WY64
User NOte:
Optimal dilutions for each application to be determined by the researcher.