ProSci

GIP Antibody

Product Code:
 
PSI-22-068
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using GIP antibody (22-068) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
2 / 5
Immunohistochemistry of paraffin-embedded rat kidney using GIP antibody (22-068) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human colon using GIP antibody (22-068) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using GIP antibody (22-068) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using GIP antibody (22-068). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GIP antibody (22-068) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using GIP antibody (22-068) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using GIP antibody (22-068) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GIP antibody (22-068) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using GIP antibody (22-068). Blue: DAPI for nuclear staining.

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PSI-22-068-100uL100 uL£565.00
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Further Information

Additional Names:
gastric inhibitory polypeptide, glucose-dependent insulinotropic polypeptide, incretin hormone
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-153 of human GIP (NP_004114.1).
NCBI Gene ID #:
2695
NCBI Official Name:
gastric inhibitory polypeptide
NCBI Official Symbol:
GIP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P09681
User NOte:
Optimal dilutions for each application to be determined by the researcher.