ProSci

UBE2B Antibody

Product Code:
 
PSI-22-124
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using UBE2B antibody (22-124) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded rat lung using UBE2B Antibody (22-124) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded rat kidney using UBE2B Antibody (22-124) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using UBE2B antibody (22-124) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using UBE2B Antibody (22-124) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using UBE2B Antibody (22-124) at dilution of 1:100 (40x lens).

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PSI-22-124-100uL100 uL£565.00
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Further Information

Additional Names:
Ubiquitin-conjugating enzyme E2 B, RAD6 homolog B, HR6B, hHR6B, Ubiquitin carrier protein B, Ubiquitin-conjugating enzyme E2-17 kDa, Ubiquitin-protein ligase B, UBE2B, RAD6B
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2B (NP_003328.1).
NCBI Gene ID #:
7320
NCBI Official Name:
Ubiquitin-conjugating enzyme E2 B
NCBI Official Symbol:
UBE2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
P63146
User NOte:
Optimal dilutions for each application to be determined by the researcher.