ProSci

MGAT1 Antibody

Product Code:
 
PSI-22-178
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using MGAT1 antibody (22-178) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded human liver damage using MGAT1 antibody (22-178) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded human stomach using MGAT1 antibody (22-178) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using MGAT1 antibody (22-178) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using MGAT1 antibody (22-178) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MGAT1 antibody (22-178) at dilution of 1:100 (40x lens).

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PSI-22-178-100uL100 uL£565.00
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Further Information

Additional Names:
MGAT1, OTTHUMP00000161546, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase, MGAT, mannosyl (alpha-1, 3-)-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase, GNT-I, GNT-1, GLYT1, GLCT1, GLCNAC-TI, GGNT1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 156-445 of human MGAT1 (NP_001108092.1).
NCBI Gene ID #:
4245
NCBI Official Name:
mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
NCBI Official Symbol:
MGAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P26572
User NOte:
Optimal dilutions for each application to be determined by the researcher.