ProSci

MLN Antibody

Product Code:
 
PSI-22-179
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using MLN Antibody (22-179) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 5
Immunohistochemistry of paraffin-embedded human stomach using MLN antibody (22-179) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded mouse brain using MLN antibody (22-179) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse heart using MLN antibody (22-179) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using MLN antibody (22-179). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using MLN Antibody (22-179) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human stomach using MLN antibody (22-179) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MLN antibody (22-179) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using MLN antibody (22-179) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MLN antibody (22-179). Blue: DAPI for nuclear staining.

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PSI-22-179-100uL100 uL£565.00
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Further Information

Additional Names:
promotilin, prepromotilin
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-115 of human MLN (NP_002409.1).
NCBI Gene ID #:
4295
NCBI Official Name:
motilin
NCBI Official Symbol:
MLN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 13kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P12872
User NOte:
Optimal dilutions for each application to be determined by the researcher.