ProSci

OTC Antibody

Product Code:
 
PSI-23-932
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using OTC antibody (23-932) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human prostate using OTC antibody (23-932) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver damage using OTC antibody (23-932) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using OTC antibody (23-932) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human prostate using OTC antibody (23-932) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using OTC antibody (23-932) at dilution of 1:100 (40x lens).

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PSI-23-932-100uL100 uL£565.00
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Further Information

Additional Names:
OTC, OCTD
Application Note:
WB: 1:1000 - 1:4000

IHC: 1:50 - 1:100
Background:
This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human OTC (NP_000522.3).
NCBI Gene ID #:
5009
NCBI Official Name:
ornithine carbamoyltransferase
NCBI Official Symbol:
OTC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P00480
User NOte:
Optimal dilutions for each application to be determined by the researcher.