K29-linked Tetra-Ubiqutin (human) (rec.) (untagged)

Ub4

Lot dependent.

32160000

liquid

Liquid. In 50mM HEPES pH 7.5.

Aliquot to avoid freeze/thaw cycles.

Recombinant Protein. Two enzymatically conjugated human ubiquitin (aa1-76) covalently linked through an isopeptide bond at K29 residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule and untagged. Source/Host: E. coli. Liquid. In 50mM HEPES pH 7.5. The array of cellular processes initiated and regulated by ubiquitin has been partially explained by the structural diversity of differently linked ubiquitin chains. In a ubiquitin chain, ubiquitin moieties can be conjugated through one of their lysine residues (K6, K11, K27, K29, K33, K48 and K63) or the N-terminal methionine residue (M1), offering countless possibilities to assemble a specific polymer. Ubiquitin molecules can also be modified by other post-translational modifications, including acetylation and phosphorylation, adding another layer of ubiquitin signal regulation and diversification. K29-linked polyubiquitination has been shown to be an inhibitor of Wnt signaling, which plays an important role in embryogenesis, and its deregulation has been shown to play a role in tumorigenesis. This protein is formed with wild-type human recombinant ubiquitin and linkage-specific enzymes. Ideal for investigating ubiquitin-binding proteins and as substrates for ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application.

~34kDa

P0CG47

Plastic Vial

The array of cellular processes initiated and regulated by ubiquitin has been partially explained by the structural diversity of differently linked ubiquitin chains. In a ubiquitin chain, ubiquitin moieties can be conjugated through one of their lysine residues (K6, K11, K27, K29, K33, K48 and K63) or the N-terminal methionine residue (M1), offering countless possibilities to assemble a specific polymer. Ubiquitin molecules can also be modified by other post-translational modifications, including acetylation and phosphorylation, adding another layer of ubiquitin signal regulation and diversification. K29-linked polyubiquitination has been shown to be an inhibitor of Wnt signaling, which plays an important role in embryogenesis, and its deregulation has been shown to play a role in tumorigenesis. This protein is formed with wild-type human recombinant ubiquitin and linkage-specific enzymes. Ideal for investigating ubiquitin-binding proteins and as substrates for ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application.

>95% (SDS-PAGE)

Two enzymatically conjugated human ubiquitin (aa1-76) (Accession Nr. P0CG47 http://www.ncbi.nlm.nih.gov/protein/P0CG47 ) covalently linked through an isopeptide bond at K29 residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule and untagged.

E. coli

no TAG

Non-hazardous

Other Proteins

12352202

Stable for at least 1 year after receipt when stored at -80°C.