ScienCell's GeneQuery™ Human Chromatin Organization and Remodeling qPCR Array Kit (GQH-CHM) profiles 88 key genes involved in regulating chromatin structure. Chromatin compacts DNA, allowing mitosis while preventing DNA damage and controlling gene expression. It is a complex macromolecule that is only found in eukaryotes consisting of RNA, DNA, and protein. The basic proteins of chromatin are histones and histone modifications contribute to altering chromatin packing and structure. Brief examples of how included genes may be grouped according to their function are shown below:
- Chromatin remodeling: - ATRX, CBX2, CREBBP, EP300, MECOM
- Chromatin organization: - HIST1H3A, HIST1H3J, HIST2H3A, HIST2H3D, HIST3H3
- Histone acetylation/deacetylation: - ELP3, HDAC1, HDAC4, JADE2, KAT6A
- Histone methylation/demethylation: - DNMT3A, DOT1L, EZH2, JMJD6, KDM6A
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
This kit profiles 88 key genes involved in the establishment and progression of human nerve tissue tumors. This kit focuses on brain tumors but also includes genes associated with neuroblastomas.
