ProSci

SARS-CoV-2 (COVID-19) S1 RBD Antibody [T5P4-A12]

Product Code:
 
PSI-SD9439
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Llama
Antibody Isotype:
 
sdAb
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
T5P4-A12
Regulatory Status:
 
RUO
Target Species:
 
Virus
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Dry Ice
Storage:
 
SARS-CoV-2 (COVID-19) S1 RBD antibody should be stored in working aliquots at -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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<strong>Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants </strong><br> Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1 μg/mL and each of the 6 anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, SD9441, and SD9503 bind spike proteins of omicron variant, but not delta variant; SD9511 and SD9437 bind spike proteins of delta variant, but not omicron variant; SD9507 binds spike proteins of delta and omicron variants.
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<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD, S1 and ECD Proteins </strong><br>Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, SD9439. A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, 10-303; S1, 97-087; ECD, 11-070) as coating antigen at 1 μg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (SD9439) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, SD9439 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.
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<strong>Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) Spike Trimer Proteins of Omicron and Delta Variants </strong><br> Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike Trimer recombinant proteins of Omicron and Delta variants as coating antigen at 1 μg/mL and each of the 6 anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SD9439, SD9441, and SD9503 bind spike proteins of omicron variant, but not delta variant; SD9511 and SD9437 bind spike proteins of delta variant, but not omicron variant; SD9507 binds spike proteins of delta and omicron variants.
<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD, S1 and ECD Proteins </strong><br>Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, SD9439. A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, 10-303; S1, 97-087; ECD, 11-070) as coating antigen at 1 μg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (SD9439) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, SD9439 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.

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CodeSizePrice
PSI-SD9439-0.02mg0.02mg£166.00
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PSI-SD9439-0.1mg0.1mg£531.00
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Further Information

Additional Names:
SARS-CoV-2 (COVID-19) Spike S1 Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).
Background References:
  • Gorbalenya. bioRxiv: 2020.
  • Hui et al. Int J Infect Dis. 2020;91:264-266.
  • Belouzard et al. Viruses. 2012;4(6):1011-33.
  • Lee et al. J Virol. 2006;80(8):4079-87.
  • Wan et al. J Virol. 2020.
  • Wrapp et al. Science. 2020.
Buffer:
SARS-CoV-2 (COVID-19) S1 RBD Antibody [T5P4-A12] Antibody is supplied in PBS.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SARS-CoV-2 S protein RBD containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Arg 319 - Lys 537.
NCBI Gene ID #:
43740568
NCBI Official Name:
surface glycoprotein
NCBI Official Symbol:
S
NCBI Organism:
SARS-CoV-2
Physical State:
Liquid
Protein Accession #:
QHD43420
Protein GI Number:
1791269090
Purification:
SARS-CoV-2 (COVID-19) S1 RBD Antibody [T5P4-A12] is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.??It also contains a myc-tag for detection.
Research Area:
Infectious Disease,COVID-19
User NOte:
Optimal dilutions for each application to be determined by the researcher.