ProSci

Streptococcus pyogenes IdeS Recombinant Protein

Product Code:
 
PSI-11-129
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Host Type:
 
E.coli
Regulatory Status:
 
RUO
Target Species:
 
Streptococcus species (Bacteria)
Application:
 
Western Blot (WB)
Shipping:
 
Dry ice
Storage:
 
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
 

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PSI-11-129-2500U2500U£577.00
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Further Information

Additional Names:
IgG-degrading protease,Sib38,IdeS
Application Note:
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.8 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE).
Background:
IdeS from Streptococcus pyogenes, also known as Mac-1, is an endopeptidase with specificity for IgG. The bacterial protein Mac-1 adopts an alpha/beta fold, with 14 beta strands and 9 alpha helices. The N-terminal domain is made up predominantly of alpha helices, whereas the C-terminal domain consists predominantly of beta sheets. Mac 1 blocks polymorphonuclear opsonophagocytosis, inhibits the production of reactive oxygen species and contains IgG endopeptidase activity.IdeS is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F(ab?)2 and Fc/2 fragments. IdeS digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG. It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species. IdeS is cloned from Streptococcus pyogenes and expressed in E. coli.
Background References:
  • Wenig K, Chatwell L, et al. Proc. Natl. Acad. Sci. U.S.A. 101, 17371-6, (2004).
  • Hulting G, Flock M, et al. FEMS Microbiol. Lett. 298, 44-50, (2009).
  • Seele J, Singpiel A, et al. J. Bacteriol. 195, 930-40, (2013).
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
Physical State:
Liquid
Predicted Molecular Weight:
36.8 kDa
Protein Accession #:
WP_010922160.1
Purity:
>95% as determined by SDS-PAGE.
Recombinant Protein Sequence:
#N/A
Source:
E. coli

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