NSJ Bioreagents

Bromodeoxyuridine / BrdU Antibody

Product Code:
 
NSJ-V2321
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BRD469
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD469).~
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IHC staining of mouse intestine tissue (20X) with BrdU antibody (BRD469).
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IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD469).~
IHC staining of mouse intestine tissue (20X) with BrdU antibody (BRD469).

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CodeSizePrice
NSJ-V2321-20UG20 ug£264.00
Quantity:
NSJ-V2321-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine antibody to be titered up or down for optimal performance.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.

Description:
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
Not Applicable
Immunogen:
Bromodeoxyuridine conjugated to KLH was used as the immunogen.
Limitation:
This BrdU antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
All species