NSJ Bioreagents

CD81 Antibody

Product Code:
 
NSJ-V7501
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
C81/2885R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant CD81 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using recombinant CD81 antibody (clone C81/2885R). Gray=unstained cells, Orange = recombinant CD81 antibody.~
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Flow cytometry staining of human MCF7 cells with recomb
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Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using recombinant CD81 antibody (clone C81/2885R). Gray=unstained cells, Orange = recombinant CD81 antibody.~
Flow cytometry staining of human MCF7 cells with recomb

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CodeSizePrice
NSJ-V7501-20UG20 ug£264.00
Quantity:
NSJ-V7501-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
ELISA (order BSA/sodium azide-free format for coating),Immunohistochemistry: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells
Application Note:
Optimal dilution of the recombinant CD81 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant full length human protein was used as the immunogen for the recombinant CD81 antibody.
Limitation:
This recombinant CD81 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P60033