NSJ Bioreagents

E-Cadherin Antibody / CDH1

Product Code:
 
NSJ-V3565
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
CDH1/2208R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone CDH1/2208R, green) and Reddot nuclear stain (red).
2 / 3
Formalin-fixed, paraffin-embedded human colon carcinoma stained with recombinant E-Cadherin antibody (clone CDH1/2208R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant E-Cadherin antibody (clone CDH1/2208R) as confirmation of integrity and purity.
❮❯

Immunofluorescent staining of human MCF7 cells with recombinant E-Cadherin antibody (clone CDH1/2208R, green) and Reddot nuclear stain (red).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with recombinant E-Cadherin antibody (clone CDH1/2208R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant E-Cadherin antibody (clone CDH1/2208R) as confirmation of integrity and purity.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V3565-20UG20 ug£264.00
Quantity:
NSJ-V3565-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant E-Cadherin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Cadherins behave as both receptors and ligands for other molecules. During development, their behavior assists in properly positioning cells: they are responsible for the separation of the different tissue layers, and for cellular migration. In the very early stages of development, E-cadherin (epithelial cadherin) is most greatly expressed. The E-cadherin-catenin complex plays a key role in cellular adhesion; loss of this function has been associated with greater tumour metastasis. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant E-Cadherin antibody.
Limitation:
This recombinant E-Cadherin antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P12830