NSJ Bioreagents

CDK1 Antibody

Product Code:
 
NSJ-V3040
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CDK1/873
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the CDC2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of formalin-fixed, paraffin-embedded human tonsil with CDC2 antibody (CDK1/873). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.~
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IHC testing of formalin-fixed, paraffin-embedded human tonsil with CDC2 antibody (CDK1/873). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.~

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CodeSizePrice
NSJ-V3040-20UG20 ug£264.00
Quantity:
NSJ-V3040-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the CDC2 antibody should be determined by the researcher.
Description:
Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. Cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated Cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the CDC2 antibody.
Limitation:
This CDC2 antibody is available for research use only.
Localization:
Nuclear and cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P06493