NSJ Bioreagents

CD6 Antibody

Product Code:
 
NSJ-V7028
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CLDA6-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Immunofluorescent staining of human MOLT4 cells with CD6 antibody (green, clone CLDA6-1) and Reddot nuclear stain (red).~
2 / 2
IHC analysis of human tonsil and CD6 antibody (clone CLDA6-1). Staining of formalin-fixed tissues requires boiling
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Immunofluorescent staining of human MOLT4 cells with CD6 antibody (green, clone CLDA6-1) and Reddot nuclear stain (red).~
IHC analysis of human tonsil and CD6 antibody (clone CLDA6-1). Staining of formalin-fixed tissues requires boiling

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CodeSizePrice
NSJ-V7028-20UG20 ug£264.00
Quantity:
NSJ-V7028-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD6 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular 'scavenger receptor cysteine-rich' (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. antibody to CD6 can be used to deplete T cells from bone marrow transplants to prevent graft versus host disease.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
923
Immunogen:
Human recombinant protein was used as the immunogen for this CD6 antibody.
Limitation:
This CD6 antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human