NSJ Bioreagents

GLO1 Antibody / Glyoxalase I

Product Code:
 
NSJ-V7812
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CPTC-GLO1-3
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Glyoxalase 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
IHC testing of FFPE human thyroid carcinoma stained with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2 / 4
IHC testing of FFPE human prostate carcinoma stained with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 4
Western blot testing of human HeLa and HepG2 cell lysate with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Predicted molecular weight ~21 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free Glyoxalase 1 antibody (clone CPTC-GLO1-3) as confirmation of integrity and purity.
❮❯

IHC testing of FFPE human thyroid carcinoma stained with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human prostate carcinoma stained with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human HeLa and HepG2 cell lysate with Glyoxalase 1 antibody (clone CPTC-GLO1-3). Predicted molecular weight ~21 kDa.
SDS-PAGE analysis of purified, BSA-free Glyoxalase 1 antibody (clone CPTC-GLO1-3) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V7812-20UG20 ug£264.00
Quantity:
NSJ-V7812-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
GLO1 is an enzyme involved in the detoxification of methylgyoxal, a byproduct of glycolysis. GLO1 expression has been demonstrated by several studies to be upregulated in various human malignant tumors, including metastatic melanoma and lung carcinoma, and thus is a target for pharmaceutical development.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Full length human recombinant protein was used as the immunogen for the Glyoxalase 1 antibody.
Limitation:
This Glyoxalase 1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q04760