NSJ Bioreagents

CD44 Antibody / HCAM

Product Code:
 
NSJ-V3012
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DF1485
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with CD44 antibody (clone DF1485).~
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Western blot testing of human U937 cells with CD44 antibody (clone DF1485). Predicted molecular weight: 80-95 kDa.
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IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with CD44 antibody (clone DF1485).~
Western blot testing of human U937 cells with CD44 antibody (clone DF1485). Predicted molecular weight: 80-95 kDa.

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CodeSizePrice
NSJ-V3012-20UG20 ug£264.00
Quantity:
NSJ-V3012-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD44 antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Recognizes a cell surface glycoprotein of 80-95kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Purified CD44 antigen (PGp-1) from lymphocyte membrane was used as the immunogen for the CD44 antibody.
Limitation:
This CD44 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse
Uniprot #:
P16070