NSJ Bioreagents

HER2 Antibody / ErbB2

Product Code:
 
NSJ-V2110
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
HRB2/282
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Store the HER2 ErbB2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 ErbB2 antibody (clone HRB2/282). These results demonstrate the foremost specificity of the HRB2/282 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 ErbB2 antibody (clone HRB2/282, green) and Reddot nuclear stain (red).
3 / 3
Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 ErbB2 antibody (clone HRB2/282); Red=isotype control, Blue= HER2 ErbB2 antibody.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 ErbB2 antibody (clone HRB2/282). These results demonstrate the foremost specificity of the HRB2/282 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 ErbB2 antibody (clone HRB2/282, green) and Reddot nuclear stain (red).
Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 ErbB2 antibody (clone HRB2/282); Red=isotype control, Blue= HER2 ErbB2 antibody.

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CodeSizePrice
NSJ-V2110-20UG20 ug£264.00
Quantity:
NSJ-V2110-100UG100 ug£534.00
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Further Information

Application Details :
ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. This HER2 antibody binds to the extracellular/cell surface region of the protein.

Description:
This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. HER2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. HER2 / ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
2064
Immunogen:
Recombinant human HER2 protein was used as the immunogen for this antibody.(1)
Limitation:
This HER2 / ErbB2 antibody is available for research use only.
Localization:
Extracellular/Intracellular cell membrane (this mAb is binds to the extracellular portion of the protein)
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human