NSJ Bioreagents

CD63 Antibody / LAMP-3

Product Code:
 
NSJ-V8259
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LAMP3/2789
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the CD63 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 5
IHC staining of FFPE human melanoma with CD63 antibody (clone LAMP3/2789). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human melanoma with CD63 antibody (clone LAMP3/2789). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of human spleen lysate with CD63 antibody (clone LAMP3/2789). Predicted molecular weight: 25-60 kDa depending on glycosylation level.
4 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD63 antibody (clone LAMP3/2789). These results demonstrate the foremost specificity of the LAMP3/2789 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
5 / 5
SDS-PAGE analysis of purified, BSA-free CD63 antibody (clone LAMP3/2789) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human melanoma with CD63 antibody (clone LAMP3/2789). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human melanoma with CD63 antibody (clone LAMP3/2789). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human spleen lysate with CD63 antibody (clone LAMP3/2789). Predicted molecular weight: 25-60 kDa depending on glycosylation level.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD63 antibody (clone LAMP3/2789). These results demonstrate the foremost specificity of the LAMP3/2789 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CD63 antibody (clone LAMP3/2789) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V8259-20UG20 ug£264.00
Quantity:
NSJ-V8259-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amino acids 100-197) was used as the immunogen for the CD63 antibody.
Limitation:
This CD63 antibody is available for research use only.
Localization:
Cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse
Uniprot #:
P08962