NSJ Bioreagents

Neurofilament Antibody (-Heavy) / NF-H / NEFH

Product Code:
 
NSJ-V7413
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
NFHP-1R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant NF-H antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of human brain lysate with recombinant NF-H antibody (clone NFHP-1R). Expected molecular weight ~200 kDa.~
2 / 2
IHC staining of FFPE human cerebellum with recombinant NF-H antibody (clone NFHP-1R). Required HIER: boil tis
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Western blot testing of human brain lysate with recombinant NF-H antibody (clone NFHP-1R). Expected molecular weight ~200 kDa.~
IHC staining of FFPE human cerebellum with recombinant NF-H antibody (clone NFHP-1R). Required HIER: boil tis

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CodeSizePrice
NSJ-V7413-20UG20 ug£264.00
Quantity:
NSJ-V7413-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant NF-H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H).
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant NF-H antibody.
Limitation:
This recombinant NF-H antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein A affinity
Species Reactivity :
Human
Uniprot #:
P12036