NSJ Bioreagents

PMEL17 / Melanoma gp100 Antibody

Product Code:
 
NSJ-V2254
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NKI-beteb
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Melanoma antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC testing of melanoma tissue stained with Melanoma antibody (NKI-beteb). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2
Analysis of HuProt(TM) microarray containing more than 19
❮❯

IHC testing of melanoma tissue stained with Melanoma antibody (NKI-beteb). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
Analysis of HuProt(TM) microarray containing more than 19

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CodeSizePrice
NSJ-V2254-20UG20 ug£264.00
Quantity:
NSJ-V2254-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Description:
By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
6490
Immunogen:
Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
Limitation:
This Melanoma gp100 antibody is available for research use only.
Localization:
Cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human