NSJ Bioreagents

Nucleophosmin Antibody / NPM1

Product Code:
 
NSJ-V7552
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NPM1/3286
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Nucleophosmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 5
IHC staining of FFPE human basal cell carcinoma with Nucleophosmin antibody (clone NPM1/3286). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 5
Immunofluorescent staining of permeabilized human K562 cells with Nucleophosmin antibody (green, clone NPM1/3286) and Phalloidin (red).
3 / 5
Flow cytometry testing of permeabilized human A549 cells with Nucleophosmin antibody (clone NPM1/3286); Red=isotype control, Blue= Nucleophosmin antibody.
4 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Nucleophosmin antibody (clone NPM1/3286). These results demonstrate the foremost specificity of the NPM1/3286 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
5 / 5
SDS-PAGE analysis of purified, BSA-free Nucleophosmin antibody (clone NPM1/3286) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human basal cell carcinoma with Nucleophosmin antibody (clone NPM1/3286). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescent staining of permeabilized human K562 cells with Nucleophosmin antibody (green, clone NPM1/3286) and Phalloidin (red).
Flow cytometry testing of permeabilized human A549 cells with Nucleophosmin antibody (clone NPM1/3286); Red=isotype control, Blue= Nucleophosmin antibody.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Nucleophosmin antibody (clone NPM1/3286). These results demonstrate the foremost specificity of the NPM1/3286 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Nucleophosmin antibody (clone NPM1/3286) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V7552-20UG20 ug£264.00
Quantity:
NSJ-V7552-100UG100 ug£534.00
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Nucleophosmin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 185-287 from the human protein was used as the immunogen for the Nucleophosmin antibody.
Limitation:
This Nucleophosmin antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P06748