NSJ Bioreagents

CD45 Antibody (Leukocyte marker)

Product Code:
 
NSJ-V8396
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PTPRC/1666
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
Store the CD45 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Flow cytometry testing of PFA-fixed human Jurkat cells with CD45 antibody (clone PTPRC/1666); Red=isotype control, Blue= CD45 antibody.~
2 / 2
Western blot testing of human spleen lysate with CD45 antibody. Expected molecular weight: 147-220 k
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Flow cytometry testing of PFA-fixed human Jurkat cells with CD45 antibody (clone PTPRC/1666); Red=isotype control, Blue= CD45 antibody.~
Western blot testing of human spleen lysate with CD45 antibody. Expected molecular weight: 147-220 k

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CodeSizePrice
NSJ-V8396-20UG20 ug£264.00
Quantity:
NSJ-V8396-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the CD45 antibody should be determined by the researcher.
Description:
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Human recombinant full-length protein was used as the immunogen for the CD45 antibody.
Limitation:
This CD45 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P08575