NSJ Bioreagents

Phosphotyrosine Antibody / P-Tyr

Product Code:
 
NSJ-V2359
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PY20
Regulatory Status:
 
RUO
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
 
Store the Phosphotyrosine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.~
2 / 2
SDS-PAGE Analysis of Purified, BSA-Free Phosphotyrosine Antibody (clone PY20). Confirmation of Integrity and Purity of th
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IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.~
SDS-PAGE Analysis of Purified, BSA-Free Phosphotyrosine Antibody (clone PY20). Confirmation of Integrity and Purity of th

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CodeSizePrice
NSJ-V2359-20UG20 ug£264.00
Quantity:
NSJ-V2359-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.

1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.

Description:
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
Not Applicable
Immunogen:
Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
Limitation:
This Phosphotyrosine antibody is available for research use only.
Localization:
Depends on phosphorylated target localization
Purity:
Protein G affinity chromatography
Species Reactivity :
NA