NSJ Bioreagents

Estrogen Receptor alpha Antibody

Product Code:
 
NSJ-V3582
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rESR1/1935
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant Estrogen Receptor alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of MCF7 cell lysate with recombinant Estrogen Receptor alpha antibody. Predicted molecular weight ~66 kDa.~
2 / 2
IHC testing of FFPE human breast cancer with Estrogen Receptor alpha antibody (clone rESR1/1935). Required H
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Western blot testing of MCF7 cell lysate with recombinant Estrogen Receptor alpha antibody. Predicted molecular weight ~66 kDa.~
IHC testing of FFPE human breast cancer with Estrogen Receptor alpha antibody (clone rESR1/1935). Required H

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CodeSizePrice
NSJ-V3582-20UG20 ug£264.00
Quantity:
NSJ-V3582-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The optimal dilution of the recombinant Estrogen Receptor alpha antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human Estrogen Receptor alpha protein was used as the immunogen for this recombinant Estrogen Receptor alpha antibody.
Limitation:
This recombinant Estrogen Receptor alpha antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P03372