NSJ Bioreagents

CD44 Antibody / HCAM

Product Code:
 
NSJ-V8251
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rHCAM/918
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
Western blot testing of human HeLa cell lysate with recombinant CD44 antibody (clone rHCAM/918). Predicted molecular weight ~81 kDa.
2 / 4
IHC staining of FFPE human tonsil tissue with recombinant CD44 antibody (clone rHCAM/918). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human cervix tissue with recombinant CD44 antibody (clone rHCAM/918). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free recombinant CD44 antibody (clone rHCAM/918) as confirmation of integrity and purity.
❮❯

Western blot testing of human HeLa cell lysate with recombinant CD44 antibody (clone rHCAM/918). Predicted molecular weight ~81 kDa.
IHC staining of FFPE human tonsil tissue with recombinant CD44 antibody (clone rHCAM/918). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervix tissue with recombinant CD44 antibody (clone rHCAM/918). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD44 antibody (clone rHCAM/918) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V8251-20UG20 ug£264.00
Quantity:
NSJ-V8251-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant full-length human CD44 protein was used as the immunogen for this recombinant CD44 antibody.
Limitation:
This recombinant CD44 antibody is available for research use only.
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human