NSJ Bioreagents

H3K9me3/S10p Antibody / Trimethyl-phospho-Histone H3 (Lys9/Ser10)

Product Code:
 
NSJ-R20236
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM162
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Store the recombinant H3K9me3/S10p antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Western blot test of acid extracts of HeLa cells (1) and recombinant Histone H3.3 (2), using recombinant H3K9me3/S10p antibody at 0.01 ug/ml, showed a band of Histone H3 modified by both trimethylation at Lysine 9 and phosphorylation at Serine 10 (K9me3/S10p) in HeLa cells.~
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Western blot test of acid extracts of HeLa cells (1) and recombinant Histone H3.3 (2), using recombinant H3K9me3/S10p antibody at 0.01 ug/ml, showed a band of Histone H3 modified by both trimethylation at Lysine 9 and phosphorylation at Serine 10 (K9me3/S10p) in HeLa cells.~

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CodeSizePrice
NSJ-R20236-25UG25ug£321.00
Quantity:
NSJ-R20236-100UG100ug£581.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.01ug/ml - 1ug/ml,ELISA: 0.01ug/ml - 0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K9me3/S10p antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant H3K9me3/S10p antibody reacts to Histone H3 only when modified at both trimethyl Lysine 9 and phospho Serine 10 (K9me3/S10p).
Format :
Purified
Formulation :
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A trimethyl-phospho-peptide corresponding to Trimethyl- Phospho-Histone H3 (Lys9/Ser10) was used as the immunogen for this recombinant H3K9me3/S10p antibody.
Limitation:
This recombinant H3K9me3/S10p antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Species Reactivity :
All Species
Uniprot #:
P84243