NSJ Bioreagents

ACR Antibody / Acrosin

Product Code:
 
NSJ-F51511
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the ACR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot analysis of ACR antibody and human T47D lysate. Predicted molecular weight ~46 kDa.
2 / 3
IHC analysis of FFPE human testis tissue stained with ACR antibody
3 / 3
ACR antibody flow cytometric analysis of human MDA-MB231 cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of ACR antibody and human T47D lysate. Predicted molecular weight ~46 kDa.
IHC analysis of FFPE human testis tissue stained with ACR antibody
ACR antibody flow cytometric analysis of human MDA-MB231 cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F51511-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51511-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:250-1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Description:
Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 99-127 from the human protein was used as the immunogen for this ACR antibody.
Limitation:
This ACR antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P10323