NSJ Bioreagents

Caspase-3 Antibody

Product Code:
 
NSJ-F41376
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the Caspase-3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Caspase-3 antibody western blot analysis in MDA-MB435 lysate
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Caspase-3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue.
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Caspase-3 antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Caspase-3 antibody western blot analysis in MDA-MB435 lysate
Caspase-3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue.
Caspase-3 antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F41376-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41376-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 219-248 from the human protein was used as the immunogen for this Caspase-3 antibody.
Limitation:
This Caspase-3 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Rat, Primate, Rabbit
Uniprot #:
P42574