NSJ Bioreagents

AMPK Antibody

Product Code:
 
NSJ-F40150
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the AMPK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot analysis of AMPK antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA1 gene (2).
2 / 3
IHC analysis of FFPE human breast carcinoma tissue stained with AMPK antibody
3 / 3
Western blot testing of human K562 cell lysate with AMPK antibody. Predicted molecular weight ~64 kDa.
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Western blot analysis of AMPK antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA1 gene (2).
IHC analysis of FFPE human breast carcinoma tissue stained with AMPK antibody
Western blot testing of human K562 cell lysate with AMPK antibody. Predicted molecular weight ~64 kDa.

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CodeSizePrice
NSJ-F40150-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40150-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the AMPK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 474-502 from human PRKAA1 was used as the immunogen for this AMPK antibody.
Limitation:
This AMPK aantibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q13131