NSJ Bioreagents

Nucleolin Antibody

Product Code:
 
NSJ-F41619
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
The Nucleolin antibody should be aliquoted and and stored frozen at -20oC. Avoid repeat freeze-thaws.
1 / 4
Nucleolin antibody western blot analysis in HepG2 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.
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Western blot analysis of Nucleolin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the NCL gene.
3 / 4
Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue.
4 / 4
Fluorescent confocal image of HeLa cell stained with Nucleolin antibody at 1:25. NCL immunoreactivity is localized to the nucleus.
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Nucleolin antibody western blot analysis in HepG2 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.
Western blot analysis of Nucleolin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the NCL gene.
Nucleolin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue.
Fluorescent confocal image of HeLa cell stained with Nucleolin antibody at 1:25. NCL immunoreactivity is localized to the nucleus.

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CodeSizePrice
NSJ-F41619-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41619-0.4ML0.4 ml£502.00
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Due to differences in protocols and secondary/substrate sensitivity, it may be necessary to titrate the Nucleolin antibody for optimal performance.
Description:
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [Wiki]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
The amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein.
Limitation:
This Nucleolin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Primate
Uniprot #:
P19338