NSJ Bioreagents

MGMT Antibody

Product Code:
 
NSJ-F50325
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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MGMT antibody western blot analysis in Jurkat lysate.
2 / 3
IHC analysis of FFPE human placenta with MGMT antibody
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Flow cytometric analysis of CEM cells using MGMT antibody (bottom histogram) compared to a <a href=../tds/rabbit-igg-isotype-control-polyclonal-antibody-n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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MGMT antibody western blot analysis in Jurkat lysate.
IHC analysis of FFPE human placenta with MGMT antibody
Flow cytometric analysis of CEM cells using MGMT antibody (bottom histogram) compared to a <a href=../tds/rabbit-igg-isotype-control-polyclonal-antibody-n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F50325-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50325-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. This protein repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the protein is irreversibly inactivated.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 156-182 from the human protein was used as the immunogen for this MGMT antibody.
Limitation:
This MGMT antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P16455