NSJ Bioreagents

PBK Antibody / TOPK

Product Code:
 
NSJ-F54731
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the TOPK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC testing of FFPE rat testis tissue with TOPK antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of mouse testis tissue lysate with TOPK antibody. Predicted molecular weight ~36 kDa.
3 / 3
Western blot testing of human HeLa tissue lysate with TOPK antibody. Predicted molecular weight ~36 kDa.
❮❯

IHC testing of FFPE rat testis tissue with TOPK antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse testis tissue lysate with TOPK antibody. Predicted molecular weight ~36 kDa.
Western blot testing of human HeLa tissue lysate with TOPK antibody. Predicted molecular weight ~36 kDa.

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CodeSizePrice
NSJ-F54731-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54731-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the TOPK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity). [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 58-86 from the mouse protein was used as the immunogen for the TOPK antibody.
Limitation:
This TOPK antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9JJ78