NSJ Bioreagents

HSC70 Antibody / HSPA8

Product Code:
 
NSJ-F54783
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the HSPA8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of 1) human A431, 2) human HeLa, 3) mouse NIH 3T3 and 4) rat H-4-II-E cell lysate with HSPA8 antibody. Expected molecular weight: 70-73 kDa.
2 / 4
IHC testing of FFPE human brain tissue with HSPA8 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human A2058 cells with HSPA8 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of human HeLa cells with HSPA8 antibody; Blue=isotype control, Green= HSPA8 antibody.
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Western blot testing of 1) human A431, 2) human HeLa, 3) mouse NIH 3T3 and 4) rat H-4-II-E cell lysate with HSPA8 antibody. Expected molecular weight: 70-73 kDa.
IHC testing of FFPE human brain tissue with HSPA8 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human A2058 cells with HSPA8 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of human HeLa cells with HSPA8 antibody; Blue=isotype control, Green= HSPA8 antibody.

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CodeSizePrice
NSJ-F54783-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54783-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the HSPA8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 82-110 from the human protein was used as the immunogen for the HSPA8 antibody.
Limitation:
This HSPA8 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P11142