NSJ Bioreagents

Cathepsin E Antibody / CTSE

Product Code:
 
NSJ-F54422
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the CTSE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Western blot testing of 1) mouse stomach and 2) rat stomach lysate with CTSE antibody. Predicted molecular weight ~43 kDa.
2 / 5
Western blot testing of 1) mouse spleen and 2) human MKN45 lysate with CTSE antibody. Predicted molecular weight ~43 kDa.
3 / 5
Western blot testing of human 1) MGC803 and 2) MKN45 lysate with CTSE antibody. Predicted molecular weight ~43 kDa.
4 / 5
IHC testing of FFPE human lung carcinoma tissue with CTSE antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human K562 cells with CTSE antibody; Blue=isotype control, Green= CTSE antibody.
❮❯

Western blot testing of 1) mouse stomach and 2) rat stomach lysate with CTSE antibody. Predicted molecular weight ~43 kDa.
Western blot testing of 1) mouse spleen and 2) human MKN45 lysate with CTSE antibody. Predicted molecular weight ~43 kDa.
Western blot testing of human 1) MGC803 and 2) MKN45 lysate with CTSE antibody. Predicted molecular weight ~43 kDa.
IHC testing of FFPE human lung carcinoma tissue with CTSE antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with CTSE antibody; Blue=isotype control, Green= CTSE antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-F54422-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54422-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the CTSE antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CTSE is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 157-187 from the human protein was used as the immunogen for the CTSE antibody.
Limitation:
This CTSE antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14091